Determination of phytocomponents and validation of squalene in ethanolic extract of Clerodendrum serratum Linn roots—using gas chromatography-mass spectroscopy and GC-FID technique

Abstract Background Clerodendrum serratum Linn commonly known as Bharangi in India has wide applications the Ayurveda and Siddha system of medicine which been attributed to treatment various diseases like asthma, cough, fever, rheumatism, cephalalgia ophthalmia. Squalene nutritional, medicinal, pharmaceutical health benefits, hence possess antioxidant cytoprotective effects. Method The study presents GC-MS analysis phytoconstituents present roots further estimation one constituents, i.e., squalene is ought be per mass spectral data obtained. was determined from ethanolic extract C. using GC-FID without derivatization. Results Four major squalene, methyl palmitate, hexadecenoic acid, stigmasterol were detected by GC-MS. eluted at 17.5min confirmed with standard peak same retention time. linearity range chosen 5–30ug/mL, method found pretty linear ( R =0.995), accurate satisfactory repeatability. Hence, phytochemical compounds validated according ICH guidelines. Conclusion Thus, green gas chromatographic can used for quantification qualification active constituents . In addition, presence metabolite developed simple, fast, environmentally friendly.